33342 pi double fluorescence staining kit (Beijing Solarbio Science)
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Beijing Solarbio Science
33342 pi double fluorescence staining kit
33342 Pi Double Fluorescence Staining Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 338 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33342+pi+double+fluorescence+staining+kit/Hoechst+33342%2FPI+Double+Stain+Kit/pmc12930366-124-2-10
Average 96 stars, based on 338 article reviews
33342 Pi Double Fluorescence Staining Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 338 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33342+pi+double+fluorescence+staining+kit/Hoechst+33342%2FPI+Double+Stain+Kit/pmc12930366-124-2-10
Average 96 stars, based on 338 article reviews
33342 pi double fluorescence staining kit - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "Puerarin attenuates myocardial ischemia-reperfusion injury by inhibiting myocardium pyroptosis via the NRF2/HO-1 signaling pathway"
Article Title: Puerarin attenuates myocardial ischemia-reperfusion injury by inhibiting myocardium pyroptosis via the NRF2/HO-1 signaling pathway
Journal: Molecular Medicine Reports
doi: 10.3892/mmr.2026.13824
Figure Legend Snippet: Pue alleviates cardiomyocyte pyroptosis via the NRF2/HO-1 signaling pathway. (A) Hoechst 33342/PI staining detected the apoptotic state of H9C2 cells (magnification, ×400) (n=3). (B) Quantitative analysis of PI-positive cells. (C) Western blotting of nuclear NRF2 expression in each group (n=3). (D) Western blotting images of key pyroptotic proteins and HO-1 in each treatment group (n=3). (E) Semi-quantification of the expression of pyroptotic proteins and HO-1 in each group of cells. (F) Semi-quantification of NRF2 protein expression. ****P<0.0001 vs. OE-NC group; ### P<0.001 and #### P<0.0001 vs. H 2 O 2 + OE-NC group. Pue, puerarin; OE, overexpression; NC, negative control; NRF2, nuclear factor E2-related factor 2; ASC, apoptosis-associated speck-like protein; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D; HO-1, heme oxygenase-1.
Techniques Used: Staining, Western Blot, Expressing, Over Expression, Negative Control, Binding Assay
Related Articles
Fluorescence:Article Title: Puerarin attenuates myocardial ischemia-reperfusion injury by inhibiting myocardium pyroptosis via the NRF2/HO-1 signaling pathway Article Snippet: Once the reaction terminated, the absorbance was measured immediately at a wavelength of 450 nm using an enzyme detector (cat. no. MK3; Thermo Fisher Scientific, Inc.) to calculate LDH activity in the supernatant. .. The Hoechst Staining:Article Title: Puerarin attenuates myocardial ischemia-reperfusion injury by inhibiting myocardium pyroptosis via the NRF2/HO-1 signaling pathway Article Snippet: Once the reaction terminated, the absorbance was measured immediately at a wavelength of 450 nm using an enzyme detector (cat. no. MK3; Thermo Fisher Scientific, Inc.) to calculate LDH activity in the supernatant. .. The Hoechst |